HPLC working Things To Know Before You Buy
HPLC working Things To Know Before You Buy
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ディテクター（検出器）としては目的とする物質の性質に応じて光学的性質（吸光度、屈折率、蛍光等）、電気化学的性質、質量分析法などを利用する装置がある。
Rotating the inner valve (proven in purple) towards the inject situation directs the cell period from the sample loop and onto the column.
예를 들어 설탕과 같이 물에 녹기 쉬운 물질을 첨가했을 때 설탕은 기름층에 거의 녹지 않으므로 물층에 많이 존재하게 됩니다. 반대로 식용유와 같이 헥산에 녹기 쉬운 용질을 첨가했을 때는 물층보다 기름층에 많이 존재합니다. 이와같이, 설탕과 식용유는 물과 헥산의 두 상 사이의 존재의 비율(=분배 비율)이 크게 다르기 때문에, 만약 당신과 이 분액깔대기에서 설탕만을 분리하고 싶다면, 분액깔대기에서 물층만을 꺼내 물을 증류시키면 설탕만을 얻을 수 있습니다.
Non-polar molecules are slowed down on their way in the column. They form different degrees of attraction While using the hydrocarbon teams principally through van der Waals dispersion forces and hydrophobic interactions.
The choice on the column kind is determined by the physicochemical Attributes in the analytes currently being separated.
The pump is accountable for offering the mobile period at a relentless movement charge. This ensures that the cellular period is continually fed into the column.
As the cellular period flows from the column, the compounds in the sample interact with the stationary stage. This interaction brings about the compounds to different based mostly on their specific properties, for example polarity, dimension, demand, or affinity.
The pump is the heart of your HPLC system. It delivers the mobile period at a relentless and high pressure (around four hundred atm) through the column. Consistent flow level is essential for acquiring high performance liquid chromatography exceptional separation and protecting reproducibility. Components to think about when picking out a circulation charge incorporate:
The figure down below demonstrates the calibration curve and calibration equation to the set of exterior criteria. Substituting the sample’s peak place in the calibration equation presents the concentration of caffeine inside the sample as 94.four mg/L.
Retention moments: The time it will require for every analyte to reach the detector, supplying a attribute fingerprint for identification.
utilizes an autosampler to inject samples. As an alternative to using read more a syringe to drive the sample in to the sample loop, the syringe draws sample into your sample loop.
The pressurized liquid is usually a mixture of solvents which include h2o, acetonitrile and/or methanol and it is often called the cell section.
, such as, has two mobile phase reservoirs that happen to be useful for an isocratic elution or a gradient elution by drawing solvents from a single or both reservoirs.
Using the Evaluation system recognized, let us handle frequent issues which could crop up and the way to troubleshoot them.